Oertli Beat

Monitoring of the Biodiversity in the small waterbodies from the Swiss National Park: potential contribution of eDNA analyses to the traditional species inventor

Project Number: CH-6756
Project Type: Permanent/Monitoring_Project
Project Duration: 00/00/0000 - ?
Funding Source: other ,
Leading Institution: HES-SO, HEPIA
Project Leader: Prof. Beat Oertli
Institut Terre-Nature-Environnement
150, route de Presinge
1254 Jussy-Genève
Phone: +41 (0)22 546 68 83
e-Mail: beat.oertli(at)hesge.ch

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Research Areas:

general biology

The objective of this master thesis is to improve the methodology used for conducting species inventories in the framework of the monitoring of the aquatic biodiversity of the small waterbodies from Macun (Swiss National Park). It will be focused on the test of eDNA analyses (metabarcoding), a promising tool that potentially can be used in all type of aquatic waterbodies, and for all types of taxonomic groups. Such method is particularly well adapted for alpine ponds, because of their closed water area (not subject to upstream contamination) and also of their low species richness (linked to altitude). Two eDNA sampling sessions will be conducted in Macun in summer 2020 and summer 2021, and targeted to the identification of the fauna: invertebrates, amphibians, fishes, mammals. The first sampling session, in 2020, will be a preliminary investigation especially useful for training on methods (field and lab methods; e.g. metabarcoding), but will also provide the first results on biodiversity. The main sampling session of 2021 will be focused on five ponds, that will be sampled with both methods: traditional methods (net-sampling) and eDNA analyses (metabarcoding). This approach will allow comparing the data obtained by both methods. Furthermore, the database on aquatic biodiversity collected by net sampling in Macun ponds between 2002 and 2019 will also be used in the comparisons. The final outcome will be a critical appraisal of the contribution of eDNA analyses for species inventories in Macun ponds. It could potentially lead to propositions for an adaptation of the sampling protocol used since 2002

Last update: 5/26/20
Source of data: ProClim- Research InfoSystem (1993-2020)
Update the data of project: CH-6756

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